Biofilm reduction, cell proliferation, anthelmintic and cytotoxicity effect of green synthesised silver nanoparticle using Artemisia vulgaris extract
- Author(s): Kiran Ejaz 1 ; Haleema Sadia 1 ; Ghazna Zia 1 ; Shabnam Nazir 1 ; Abida Raza 2 ; Shaukat Ali 1 ; Tariq Iqbal 3 ; Saiqa Andleeb 1
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View affiliations
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Affiliations:
1:
Microbial Biotechnology and Medical Toxicology Laboratory, Department of Zoology , University of Azad Jammu and Kashmir , Muzaffarabad, 13100 , Pakistan ;
2: National Institute for Lasers and Optronics (NILOP) , Pakistan Atomic Energy Commission, Islamabad , Pakistan ;
3: Department of Physics , University of Azad Jammu and Kashmir , Muzaffarabad, 13100 , Pakistan
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Affiliations:
1:
Microbial Biotechnology and Medical Toxicology Laboratory, Department of Zoology , University of Azad Jammu and Kashmir , Muzaffarabad, 13100 , Pakistan ;
- Source:
Volume 12, Issue 1,
February
2018,
p.
71 – 77
DOI: 10.1049/iet-nbt.2017.0096 , Print ISSN 1751-8741, Online ISSN 1751-875X
Infectious diseases are caused by etiological agents. Nanotechnology has been used to minimise the effect of clinical pathogens which have resistance to antibiotics. In current research synthesis, characterisation and biological activities of green synthesised nanoparticles using Artemisia vulgaris extract have been done. The characterisation of AgNPs was carried out using Fourier transform infrared spectroscopy, UV-Vis spectrophotometry, and scanning electron microscopy. Anti-biofilm, cell viability, antibacterial, brine shrimp lethality, and deoxyribonucleic acid protection effects have been screened. UV-Vis spectra showed the absorption peak of synthesised nanoparticles at 400 nm. FT-IR indicated the involvement of the functional group in the preparation of AgNPs. SEM showed the spherical shape of AgNPs with 30 nm diameter. Biological screening results revealed the antibacterial effect against clinical bacterial pathogens. Biofilm reduction and cell viability assay also supported the antibacterial effect. Cytotoxicity effect was recorded as 100% at 200 μg/ml through brine shrimp lethality assay. Protein kinase inhibition zones recorded for AgNPs (16 mm bald) compared with A. vulgaris extract (11 mm bald). It has been concluded that green synthesised AgNPs are more effective against infectious pathogens and could be used as a potential source for therapeutic drugs.
Inspec keywords: microorganisms; silver; diseases; nanoparticles; enzymes; Fourier transform infrared spectra; nanomedicine; molecular biophysics; cellular biophysics; visible spectra; toxicology; ultraviolet spectra
Other keywords: green synthesised silver nanoparticle; anthelmintic effect; cell proliferation; cytotoxicity effect; deoxyribonucleic acid protection effects; antibiofilm; brine shrimp lethality; therapeutic drugs; biofilm reduction; Artemisia vulgaris extract; protein kinase inhibition zones; Fourier transform infrared spectroscopy; infectious diseases; cell viability; scanning electron microscopy; AgNP; aetiological agents; cytotoxicity; UV-Vis spectrophotometry; SEM
Subjects: Nanotechnology applications in biomedicine; Cellular biophysics; Biomedical engineering; Interactions with radiations at the biomolecular level
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