access icon free Comparison effects of titanium dioxide nanoparticles on immune cells in adaptive and innate immune system

© The Institution of Engineering and Technology
Received 13/10/2016, Accepted 08/05/2017, Revised 09/02/2017, Published 12/05/2017

Titanium dioxide nanoparticles (TiO2-NPs) have been increasingly mixed in food and daily use products. Therefore, the investigation of cytotoxic effects of TiO2-NPs is required to allay concerns of health effects related to contact with products containing TiO2-NPs. In this study, the authors demonstrated how TiO2-NPs impact on two main sub-types of immune cells that play a major role in adaptive and innate immune system. Human T-lymphocytes (Jurkat cells) and murine macrophages (RAW 264.7 cells) were used in this study. The authors results showed that cell viability of Jurkat and RAW 264.7 cells were significantly decreased, when cells were treated with TiO2-NPs at 250 and 500 µg/ml. However, the decrease of cell viability of RAW 264.7 cells was higher than that of Jurkat cells. A similar trend was also found in DNA fragmentation. An induction of reactive oxygen species was detected in both cells treated with TiO2-NPs at concentrations ≥25 µg/ml. A significant induction of tumour necrosis factor alpha (TNF-α) was found in Jurkat and RAW 264.7 cells treated with 25 µg/ml TiO2-NPs. In contrast, there was no significant induction of interleukin-6 (IL-6) in both cells that were treated with different concentrations of TiO2-NPs.

Inspec keywords: tumours; toxicology; molecular biophysics; nanoparticles; molecular configurations; nanomedicine; DNA; biomedical materials; cellular biophysics; titanium compounds; blood

Other keywords: human T-lymphocytes; adaptive immune system; cell viability; cytotoxic effects; interleukin-6; DNA fragmentation; TiO2; food products; reactive oxygen species; titanium dioxide nanoparticles; RAW 264.7 cells; TNF-α; Jurkat cells; immune cells; daily use products; innate immune system; murine macrophages; tumour necrosis factor alpha; comparison effects; health effects

Subjects: Macromolecular configuration (bonds, dimensions); Biomolecular structure, configuration, conformation, and active sites; Nanotechnology applications in biomedicine; Biomedical materials; Cellular biophysics; Biomolecular interactions, charge transfer complexes

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