access icon free Biomimetic synthesis and anticancer activity of Eurycoma longifolia branch extract-mediated silver nanoparticles

© The Institution of Engineering and Technology
Received 02/09/2016, Accepted 20/06/2017, Revised 22/05/2017, Published 19/07/2017

In the present study, silver nanoparticles (AgNPs) were synthesised by adding 1 mM Ag nitrate solution to different concentrations (1%, 2.5%, 5%) of branch extracts of Eurycoma longifolia, a well known medicinal plant in South–East Asian countries. Characterisation of AgNPs was carried out using techniques such as ultraviolet–visible spectrophotometry, X-ray diffractrometry, Fourier transform infrared–attenuated total reflection spectroscopy (FTIR–ATR), scanning electron microscopy. XRD analysis revealed face centre cubic structure of AgNPs and FTIR–ATR showed that primary and secondary amide groups in combination with the protein molecules present in the branch extract were responsible for the reduction and stabilisation of AgNPs. Furthermore, antioxidant [2,2-diphenyl-1-picrylhydrazyl and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)], antimicrobial and anticancer activities of AgNPs were investigated. The highest bactericidal activity of these biogenic AgNPs was found against Escherichia coli with zone inhibition of 11 mm. AgNPs exhibited significant anticancer activity against human glioma cells (DBTRG and U87) and human breast adenocarcinoma cells (MCF-7 and MDA-MB-231) with IC50 values of 33, 42, 60 and 38 µg/ml.

Inspec keywords: nanomedicine; silver; nanofabrication; visible spectra; nanoparticles; biochemistry; X-ray diffraction; Fourier transform infrared spectra; ultraviolet spectra; cancer; proteins; biomedical materials; antibacterial activity; biomimetics; molecular biophysics; attenuated total reflection; cellular biophysics; microorganisms

Other keywords: antioxidant; X-ray diffractometry; secondary amide groups; medicinal plant; bactericidal activity; Biomimetic synthesis; scanning electron microscopy; MCF-7 human breast adenocarcinoma cells; FTIR-ATR spectroscopy; DBTRG human glioma cells; protein molecules; Eurycoma longifolia branch extract-mediated silver nanoparticles; U87 human glioma cells; 2,2-diphenyl-1-picrylhydrazyl; 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid); antimicrobial activity; Ag; ultraviolet-visible spectrophotometry; Fourier transform infrared-attenuated total reflection spectroscopy; anticancer activity; Escherichia coli; face centre cubic structure; MDA-MB-231 human breast adenocarcinoma cells; nitrate solution; XRD; primary amide groups; zone inhibition; biogenic silver nanoparticles

Subjects: Physical chemistry of biomolecular solutions and condensed states; Other methods of nanofabrication; Infrared and Raman spectra in metals; Optical properties of metals and metallic alloys (thin films, low-dimensional and nanoscale structures); Biomedical materials; Cellular biophysics; Visible and ultraviolet spectra of metals, semimetals, and alloys; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Low-dimensional structures: growth, structure and nonelectronic properties; Nanotechnology applications in biomedicine

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