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access icon free Characterisation of sol–gel method synthesised MgZnFe2O4 nanoparticles and its cytotoxic effects on breast cancer cell line, MDA MB-231 in vitro

© The Institution of Engineering and Technology
Received 08/01/2016, Accepted 17/05/2016, Revised 04/05/2016, Published 19/05/2016

In this study, nanocrystalline magnesium zinc ferrite nanoparticles were successfully prepared by a simple sol–gel method using copper nitrate and ferric nitrate as raw materials. The calcined samples were characterised by differential thermal analysis/thermogravimetric analysis, Fourier transform infrared spectroscopy and X-ray diffraction. Transmission electron microscopy revealed that the average particle size of the calcined sample was in a range of 17–41 nm with an average of 29 nm and has spherical size. A cytotoxicity test was performed on human breast cancer cells (MDA MB-231) and (MCF-7) at various concentrations starting from (0 µg/ml) to (800 µg/ml). The sample possessed a mild toxic effect toward MDA MB-231 and MCF-7 after being examined with MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide) assay for up to 72 h of incubation. Higher reduction of cells viability was observed as the concentration of sample was increased in MDA MB-231 cell line than in MCF-7. Therefore, further cytotoxicity tests were performed on MDA MB-231 cell line.

Inspec keywords: sol-gel processing; particle size; transmission electron microscopy; differential thermal analysis; zinc compounds; toxicology; nanomedicine; Fourier transform infrared spectra; cellular biophysics; calcination; magnesium compounds; biological organs; organic compounds; cancer; X-ray diffraction; nanoparticles; nanofabrication

Other keywords: human breast cancer cells; thermogravimetric analysis; average particle size; breast cancer cell line; sol-gel method; transmission electron microscopy; ferric nitrate; cell viability; nanocrystalline magnesium zinc ferrite nanoparticles; size 17 nm to 41 nm; MDA MB-231 in vitro; cytotoxic effects; MCF-7; 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay; X-ray diffraction; copper nitrate; calcined samples; mild toxic effect; cytotoxicity testing; MDA MB-231 cell line; differential thermal analysis; Fourier transform infrared spectroscopy; raw materials

Subjects: Other heat and thermomechanical treatments; Optical properties of other inorganic semiconductors and insulators (thin films, low-dimensional and nanoscale structures); Nanofabrication using thin film deposition methods; Cellular biophysics; Nanotechnology applications in biomedicine; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Deposition from liquid phases (melts and solutions); Biomedical measurement and imaging; Biomedical engineering; Infrared and Raman spectra in inorganic crystals

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