Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism

Mukesh Singh

Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism

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Author(s): Mukesh Singh ¹
- Affiliations: 1: Department of Biotechnology, Haldia Institute of Technology, HIT campus, Hatiberia, Purba Medinipur, Haldia 721 657, India
Source: Volume 10, Issue 5, October 2016, p. 276 – 280
DOI: 10.1049/iet-nbt.2015.0063 , Print ISSN 1751-8741, Online ISSN 1751-875X

Received 12/08/2015, Accepted 08/12/2015, Revised 30/11/2015, Published 01/10/2016

Elucidation of the role of silver nanoparticles (AgNPs) in combating bacterial infection is important for the development of new antimicrobial compounds. In this study, several key factors underlying biological effects of biogenic AgNPs were investigated on recombinant Escherichia coli (XL1-Blue) which contains a reporter gene encoding β-galactosidase enzyme. Biogenic AgNPs were prepared from the tea decoction. Cytotoxicity effects were profound on the bacteria tested by the synthesised NPs. The β-galactosidase activity of the released intracellular proteins in the supernatant of E. coli was used as a measure of membrane damage and cellular leakage. Occurrence of a significant amount of β-galactosiadase activity in the supernatant of treated cells clearly demonstrated the formation of holes in the bacterial membrane. Scanning electron microscope pictures visibly indicated destruction of the membrane of the bacteria, which further confirmed membrane damage. The synthesised NPs caused damage of E. coli genomic DNA in a dose dependent manner.

References

1. 1)
  - 19. Sondi, I., Sondi, B.S.: ‘Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria’, J. Colloid. Interface Sci., 2004, 275, (1), pp. 177–182 (doi: 10.1016/j.jcis.2004.02.012).
2. 2)
  - 21. Omeroglu, E.E., Karaboz, I., Sukatar, A., et al: ‘Determination of heavy metal susceptibilities of Vibrio harveyi strains by using 2,3,5-triphenyltetrazolium chloride (TTC)’, Rapp. Comm. Int. Mer Médit., 2007, 38, p. 364.
3. 3)
  - 33. Sheikpranbabu, S., Kalishwaralal, K., Lee, K., et al: ‘The inhibition of advanced glycation end-products- induced retinal vascular permeability by silver nanoparticles’, Biomaterials, 2010, 31, pp. 2260–2271 (doi: 10.1016/j.biomaterials.2009.11.076).
4. 4)
  - 5. Andersson, D.I., Hughes, D.: ‘Antibiotic resistance and its cost: is it possible to reverse resistance?’, Nat. Rev. Microbiol., 2010, 8, pp. 260–271.
5. 5)
  - 35. Sen, I.K., Mandal, A.K., Chakraborti, S., et al: ‘Green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity’, Int. J. Biol. Macromol., 2013, 62, pp. 439–449 (doi: 10.1016/j.ijbiomac.2013.09.019).
6. 6)
  - 30. Hajipour, M.J., Fromm, K.M., Ashkarran, A.A., et al: ‘Antibacterial properties of nanoparticles’, Trends Biotechnol., 2012, 30, (10), pp. 499–511 (doi: 10.1016/j.tibtech.2012.06.004).
7. 7)
  - 9. Mei, L., Lu, Z., Zhang, W., et al: ‘Bioconjugated nanoparticles for attachment and penetration into pathogenic bacteria’, Biomaterials, 2013, 34, (38), pp. 10328–10337 (doi: 10.1016/j.biomaterials.2013.09.045).
8. 8)
  - 11. Yi-Hsuan, S., Tsegaye, M., Varhue, W., et al: ‘Quantitative dielectrophoretic tracking for characterization and separation of persistent subpopulations of Cryptosporidium parvum’, Analyst, 2014, 139, pp. 66–73 (doi: 10.1039/C3AN01810E).
9. 9)
  - 6. Bridier, A., Briandet, R., Thomas, V., et al: ‘Resistance of bacterial biofilms to disinfectants: a review’, Biofouling, 2011, 27, (9), pp. 1017–1032 (doi: 10.1080/08927014.2011.626899).
10. 10)
  - 34. Sahoo, A.K., Sk, M.P., Ghosh, S.S., et al: ‘Plasmid DNA linearization in the antibacterial action of a new fluorescent Ag nanoparticle-paracetamol dimer composite’, Nanoscale, 2011, 3, (10), pp. 4226–4233 (doi: 10.1039/c1nr10389j).
11. 11)
  - 8. Agnihotri, S., Mukherji, S., Mukherji, S.: ‘Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy’, Nanoscale, 2013, 5, pp. 7328–7340 (doi: 10.1039/c3nr00024a).
12. 12)
  - 6. Prabhu, S., Poulose, E.K.: ‘Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects’, INT Nano Lett., 2012, 2, pp. 1–10 (doi: 10.1186/2228-5326-2-32).
13. 13)
  - 20. Kumar, S., Singh, M., Halder, D., et al: ‘Mechanistic study of antibacterial activity of biologically synthesized silver nanocolloids’, Colloids Surf A,: Physicochem. Eng. Aspects., 2014, 449, pp. 82–86 (doi: 10.1016/j.colsurfa.2014.02.027).
14. 14)
  - 7. Borgesa, A., Serra, S., Abreu, A.C., et al: ‘Evaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity’, Biofouling, 2014, 30, (2), pp. 183–195 (doi: 10.1080/08927014.2013.852542).
15. 15)
  - 31. Zhang, W., Li, Y., Niu, J., et al: ‘Photogeneration of reactive oxygen species on uncoated silver, gold, nickel, and silicon nanoparticles and their antibacterial effects’, Langmuir, 2013, 29, pp. 4647–4651 (doi: 10.1021/la400500t).
16. 16)
  - 29. Singh, A., Jain, D., Upadhyay, M.K., et al: ‘Green synthesis of silver nanoparticles using Argemone Mexicana leaf extract and evaluation of their antimicrobial activities’, Dig. J. Nanomater. Biostruct., 2010, 5, (2), pp. 483–489.
17. 17)
  - 24. Li, W.R., Xie, X.B., Shi, Q.S., et al: ‘Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli’, Appl. Microbiol. Biotechnol., 2010, 85, (4), pp. 1115–1122 (doi: 10.1007/s00253-009-2159-5).
18. 18)
  - 23. Lubick, N.: ‘Nanosilver toxicity: ions, nanoparticless or both?’, Environ. Sci. Technol., 2008, 42, (23), p. 8617 (doi: 10.1021/es8026314).
19. 19)
  - 25. Garcia, M.A., Venta, J., Crespo, P., et al: ‘Surface plasmon resonance of capped Au nanoparticles’, Phys. Rev. B, 2005, 72, (24), pp. 1–4 (doi: 10.1103/PhysRevB.72.241403).
20. 20)
  - 17. Baker, C., Pradhan, A., Pakstis, L., et al: ‘Synthesis and antibacterial properties of silver nanoparticles’, J. Nanosci. Nanotechnol., 2005, 5, (2), pp. 244–249 (doi: 10.1166/jnn.2005.034).
21. 21)
  - 22. Guo, D., Zhu, L., Huang, Z., et al: ‘Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions’, Biomaterials, 2013, 34, pp. 7884–7894 (doi: 10.1016/j.biomaterials.2013.07.015).
22. 22)
  - 22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., et al: ‘Protein measurement with the folin phenol reagent’, J. Biol. Chem., 1951, 193, (1), pp. 265–275.
23. 23)
  - 2. Saiman, L.: ‘Microbiology of early CF lung disease’, Paediatr. Respir. Rev., 5 Suppl. A, 2004, 5, pp. S367–S379 (doi: 10.1016/S1526-0542(04)90065-6).
24. 24)
  - 12. Bridle, H., Kersaudy-Kerhoas, M., Miller, B., et al: ‘Detection of Cryptosporidium in miniaturised fluidic devices’, Water Res., 2012, 46, pp. 1641–1661 (doi: 10.1016/j.watres.2012.01.010).
25. 25)
  - 18. Hamouda, T., Myc, A., Donovan, B., et al: ‘A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi’, Microbiol. Res., 2000, 156, pp. 1–7 (doi: 10.1078/0944-5013-00069).
26. 26)
  - 13. Faussa, E.K., MacCuspieb, R.I., Oyanedel-Craverc, V., et al: ‘Disinfection action of electrostatic versus steric-stabilized silvernanoparticles on E. coli under different water chemistries’, Colloids Surf. B, Biointerfaces, 2014, 113, pp. 77–84 (doi: 10.1016/j.colsurfb.2013.08.027).
27. 27)
  - 16. Kim, J.S., Kuk, E., Yu, K., et al: ‘Antimicrobial effects of silver nanoparticles’, Nanomedicine, 2007, 3, pp. 95–105.
28. 28)
  - 28. Zhang, H., Smith, J.A., Oyanedel-Craver, V.: ‘The effect of natural water conditions on the anti-bacterial performance and stability of silver nanoparticles capped with different polymers’, Water Res., 2012, 46, pp. 691–699 (doi: 10.1016/j.watres.2011.11.037).
29. 29)
  - 1. Brusselaers, N., Vogelaers, D., Blot, S.: ‘The rising problem of antimicrobial resistance in the intensive care unit’, Ann. Intensive Care, 2011, 1, pp. 47–53 (doi: 10.1186/2110-5820-1-47).
30. 30)
  - 19. Goswami, S.R., Sahareen, T., Singh, M., et al: ‘Role of biogenic silver nanoparticles in disruption of cell–cell adhesion in Staphylococcus aureus and Escherichia coli biofilm’, J. Ind. Eng. Chem., 2015, 26, pp. 73–80 (doi: 10.1016/j.jiec.2014.11.017).
31. 31)
  - 3. Zhang, L., Pornpattananangkul, D., Hu, C.M.J., et al: ‘Development of nanoparticles for antimicrobial drug delivery’, Curr. Med. Chem., 2010, 17, pp. 585–594 (doi: 10.2174/092986710790416290).
32. 32)
  - 4. Kang, K., Jung, H., Lim, J.S.: ‘Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling’, Biomol. Ther. (Seoul)., 2012, 20, (4), pp. 399–405 (doi: 10.4062/biomolther.2012.20.4.399).
33. 33)
  - 26. Sau, K., Rogach, A.L., Jäckel, F., et al: ‘Properties and applications of colloidal nonspherical noble metal nanoparticles’, Adv. Mater., 2010, 22, (16), pp. 1805–1825 (doi: 10.1002/adma.200902557).
34. 34)
  - 27. Vaidyanathan, R., Kalishwaralal, K., Gopalram, S., et al: ‘Nanosilver – the burgeoning therapeutic molecule and its green synthesis’, Biotechnol. Adv., 2009, 27, pp. 924–937 (doi: 10.1016/j.biotechadv.2009.08.001).

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Elucidation of biogenic silver nanoparticles susceptibility towards Escherichia coli: an investigation on the antimicrobial mechanism

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