Green synthesis of silver nanoparticles for selective toxicity towards cancer cells

Kasivelu Govindaraju; Karthikeyan Krishnamoorthy; Suliman A. Alsagaby; Ganesan Singaravelu; Mariappan Premanathan

Green synthesis of silver nanoparticles for selective toxicity towards cancer cells

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Author(s): Kasivelu Govindaraju ^{1, 2} ; Karthikeyan Krishnamoorthy ³ ; Suliman A. Alsagaby ⁴ ; Ganesan Singaravelu ² ; Mariappan Premanathan ⁴
- Affiliations: 1: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600119, India ;
  2: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632004, Tamilnadu, India ;
  3: Nanomaterials and System Lab, Department of Mechanical Engineering, Jeju National University, Jeju 690-456, Republic of Korea ;
  4: Biosciences Research Centre, Department of Medical Laboratory, College of Science, Majmaah University, Zulfi 11932, Saudi Arabia
Source: Volume 9, Issue 6, December 2015, p. 325 – 330
DOI: 10.1049/iet-nbt.2015.0001 , Print ISSN 1751-8741, Online ISSN 1751-875X

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Received 01/01/2015, Accepted 05/05/2015, Revised 30/03/2015, Published 01/12/2015

Therapeutic applications of nanoparticles (NPs) are rapidly increasing for their utility in medicine, especially cancer therapy. The present study investigated the green synthesis of silver NPs (Ag NPs) of 10 nm size using Sargassum vulgare and its preferential ability to kill cancerous human myeloblastic leukemic cells HL60 and cervical cancer cells HeLa as compared with normal peripheral blood mononuclear cells. DNA fragmentation study and annexin V marker fluorescence-activated cell sorting (FACS) analysis revealed the Ag NP-induced cell death is through apoptosis. Transmission electron micrographs have showed the endocytosis of Ag NPs into the nucleus. Ag NPs inhibited the lipid peroxidation-induced reactive oxygen species generation, thus preventing the irradiation-related carcinogenesis. This study suggested that a mechanism underlying the toxicity of Ag NPs towards cancer cells is due to DNA damage and apoptosis. The authors’ findings revealed the potential utility of as-prepared Ag NPs in the treatment of cancer as prophylactic agent with antioxidant property and chemotherapeutic agent for their selective toxicity to cancer cells.

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