Component analyses of urinary nanocrystallites of uric acid stone formers by combination of high-resolution transmission electron microscopy, fast Fourier transformation, energy dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy
- Author(s): Xin-Yuan Sun 1 ; Jun-Fa Xue 1 ; Zhi-Yue Xia 1 ; Jian-Ming Ouyang 1
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View affiliations
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Affiliations:
1:
Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China
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Affiliations:
1:
Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People's Republic of China
- Source:
Volume 9, Issue 3,
June 2015,
p.
114 – 121
DOI: 10.1049/iet-nbt.2014.0017 , Print ISSN 1751-8741, Online ISSN 1751-875X
© The Institution of Engineering and Technology
Received
17/05/2014,
Accepted
20/08/2014,
Revised
29/07/2014,
Published
02/10/2014
This study aimed to analyse the components of nanocrystallites in urines of patients with uric acid (UA) stones. X-ray diffraction (XRD), Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy (HRTEM), fast Fourier transformation (FFT) of HRTEM, and energy dispersive X-ray spectroscopy (EDS) were performed to analyse the components of these nanocrystallites. XRD and FFT showed that the main component of urinary nanocrystallites was UA, which contains a small amount of calcium oxalate monohydrate and phosphates. EDS showed the characteristic absorption peaks of C, O, Ca and P. The formation of UA stones was closely related to a large number of UA nanocrystallites in urine. A combination of HRTEM, FFT, EDS and XRD analyses could be performed accurately to analyse the components of urinary nanocrystallites.
Inspec keywords: calcium compounds; infrared spectra; nanostructured materials; carbon; calcium; transmission electron microscopy; diseases; fast Fourier transforms; oxygen; Fourier transform spectra; spectrochemical analysis; X-ray chemical analysis; phosphorus compounds; patient diagnosis; phosphorus; X-ray diffraction
Other keywords: oxygen characteristic absorption peaks; phosphates; uric acid stones; carbon characteristic absorption peaks; phosphorus characteristic absorption peaks; X-ray diffraction; calcium characteristic absorption peaks; Ca; O; calcium oxalate monohydrate; urinary nanocrystallite component analysis; energy dispersive X-ray spectroscopy; high-resolution transmission electron microscopy; fast Fourier transformation; Fourier transform infrared spectroscopy; P; C
Subjects: Electromagnetic radiation spectrometry (chemical analysis); Biomedical measurement and imaging; Patient diagnostic methods and instrumentation
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