access icon free DNA adduct identification using gold-aptamer nanoprobes

The optical and physico-chemical properties of gold nanoparticles (AuNPs) have prompted new and improved approaches which have greatly evolved the fields of biosensing and molecular detection. In this study, the authors took advantage of AuNPs’ ease of modification and functionalised it with selected DNA aptamers using a salt aging method to produce gold-aptamer nanoprobes. After characterisation, these nanoprobes were subsequently used for biomolecular detection of glycidamide (GA)–guanine (Gua) adducts generated in vitro. The results are based on differences in nanoprobe stabilisation against salt-induced aggregation, similar to the non-cross-linking method developed by Baptista for discrimination of specific sequences. Alkylated Guas were efficiently discriminated from deoxyguanosine and GA in solution. Despite this, a clear identification of DNA adducts derived from genomic DNA alkylation has proven to be a more challenging task.

Inspec keywords: gold; biochemistry; molecular configurations; nanomedicine; DNA; aggregation; genomics; molecular biophysics; proteins

Other keywords: DNA adduct identification; genomic DNA alkylation; biosensor; Au; biomolecular detection; physico-chemical properties; salt-induced aggregation; optical properties; nanoprobe stabilisation; gold nanoparticles; DNA aptamers; gold-aptamer nanoprobes; in vitro glycidamide-guanine adduct generation; deoxyguanosine; specific sequences; Gua alkylation; salt aging

Subjects: Physical chemistry of biomolecular solutions and condensed states; Biomedical measurement and imaging; Nanotechnology applications in biomedicine; Biomolecular structure, configuration, conformation, and active sites; Macromolecular constitution (chains and sequences); Physics of subcellular structures; Biomolecular dynamics, molecular probes, molecular pattern recognition

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http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2014.0007
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