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access icon free Interactions between gold nanoparticles and amyloid β 25–35 peptide

Amyloid β 25–35 ( 25–35) peptide is a peculiar peptide for its rapid aggregation properties and high neurotoxicity in Alzheimer's disease. Here, the interactions between gold nanoparticles (GNPs) and 25–35 monomers, oligomers and fibrils are explored under different molar ratio, temperature and pH by ultraviolet–visible and circular dichroism spectra, thioflavin T fluorescence assay and transmission electron microscope. It is concluded that 25–35 can induce the aggregation of GNPs at certain concentration of 25–35 monomer or oligomer. But at higher concentration of 25–35, GNPs aggregates dissociate again. Furthermore, the aggregation rate increases at higher temperature or for lower pH. These results might provide the basis of a simple diagnostic tool for detecting Alzheimer's disease.

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http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2013.0071
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