access icon free Green rapid biogenic synthesis of bioactive silver nanoparticles (AgNPs) using Pseudomonas aeruginosa

The present work was focused on isolating a bacterial strain of Pseudomonas sp. with the ability to synthesise AgNPs rapidly. A strain of Pseudomonas aeruginosa designated JO was found to be a potential candidate for rapid synthesis of AgNPs with a synthesis time of 4h in light, at room temperature which is a shorter time period noticed for the synthesis when compared to the previous reports Biosynthesis of AgNPs was achieved by addition of culture supernatant with aqueous silver nitrate solution (1 mM). The reaction mixture exhibits change in colour from green to brown with a peak at 420 nm corresponding to the plasmon absorbance of AgNPs by UV–vis spectroscopy. The nanoparticles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray analysis, Fourier-transform infrared spectroscopy, scanning electron microscopy (SEM), Zetasizer and transmission electron microscopy (TEM). The XRD spectrum exhibited 2θ values corresponding to the silver nanocrystals. TEM and SEM micrographs revealed the extracellular formation of polydispersed elongated nanoparticles with an average size of 27.5 nm. Synthesised nanoparticles showed antibacterial property against both gram-positive and gram-negative microorganisms, but more effective towards gram-negative.

Inspec keywords: antibacterial activity; plasmons; biomedical materials; nanofabrication; Fourier transform spectra; microorganisms; infrared spectra; nanomedicine; X-ray chemical analysis; visible spectra; transmission electron microscopy; scanning electron microscopy; ultraviolet spectra; nanoparticles; X-ray diffraction; silver

Other keywords: Zetasizer; aqueous silver nitrate solution; polydispersed elongated nanoparticles; UV-vis spectroscopy; antibacterial property; transmission electron microscopy; Fourier-transform infrared spectroscopy; bioactive silver nanoparticles; TEM; room temperature; XRD; gram-positive microorganisms; gram-negative microorganisms; culture supernatant; Ag; green rapid biogenic synthesis; energy-dispersive X-ray analysis; bacterial strain; X-ray diffraction; SEM; scanning electron microscopy; biosynthesis; Pseudomonas aeruginosa; plasmon absorbance

Subjects: Nanotechnology applications in biomedicine; Biomedical materials; Electromagnetic radiation spectrometry (chemical analysis); Optical properties of metals and metallic alloys (thin films, low-dimensional and nanoscale structures); Methods of nanofabrication and processing; Infrared and Raman spectra in metals; Visible and ultraviolet spectra of metals, semimetals, and alloys

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