access icon free Sensitive electrochemical biosensing of H2O2 based on cobalt nanoparticles synthesised in iron storage protein molecules, ferritin

In this report, a highly sensitive electrochemical biosensor based on cobaltferritin immobilised on a self-assembled monolayer modified gold electrode for determination of hydrogen peroxide (H2O2) in phosphate buffer solution (pH 7.5) was investigated. The modified electrode showed excellent electrochemical activity for oxidation of H2O2. The response to H2O2 on the modified electrode was examined using linear sweep and differential pulse voltammetries. In phosphate buffer (pH 7.5, 0.1 M), the fabricated biosensor exhibited a linear dependence (R = 0.989) on the concentration of H2O2 from 2.49 × 10−9 to 1.91 × 10−8 M, a high sensitivity of −0.4099 µA/nM and detection limit of 2.48 × 10−9 based on a signal-to-noise ratio of 3. Charge transfer coefficient (α) and the exchange current (i 0) of oxidation for H2O2 were found to be 0.57 and 7.55 A, respectively. It has been shown that, this modified electrode is able to determine H2O2 with a high sensitivity, low detection limit and high selectivity.

Inspec keywords: nanosensors; electrochemical electrodes; pH; voltammetry (chemical analysis); electrochemical sensors; monolayers; oxidation; nanoparticles; self-assembly; gold; hydrogen compounds; nanobiotechnology; cobalt; biosensors; biochemistry

Other keywords: electrochemical activity; current 0.57 A; cobalt nanoparticles; Co; low detection limit; high selectivity; differential pulse voltammetry; oxidation; biosensor; pH; iron storage protein molecules; high sensitivity; current 7.55 A; charge transfer coefficient; phosphate buffer solution; H2O2; self-assembled monolayer; sensitive electrochemical biosensing; ferritin; exchange current; electrode; Au; linear sweep voltammetry

Subjects: Microsensors and nanosensors; Specific chemical reactions; reaction mechanisms; Electrochemical analytical methods; Chemical sensors; Electrochemistry and electrophoresis; Biosensors; Chemical variables measurement; Chemical sensors; Biological engineering and techniques; Biosensors; Physical chemistry of biomolecular solutions and condensed states

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
    8. 8)
    9. 9)
      • 27. Bard, A.J., Faulkner, L.R.: ‘Electrochemical methods fundamentals and applications’ (John Wiley, 2001, 2nd edn.).
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)
    18. 18)
      • 28. Ichiro, Y.: ‘Method for preparing cobalt and minus; protein complex’. European Patent 1433743A1, June2004.
    19. 19)
    20. 20)
    21. 21)
    22. 22)
    23. 23)
    24. 24)
    25. 25)
    26. 26)
      • 23. Nezhad, G.K., Pashazadeh, S., Pashazadeh, A.: ‘Ni/Al LDH nanoparticles modified carbon paste electrode: application to electro-catalytic oxidation of methanol’, Anal. Bioanal. Electrochem., 2012, 4, (4), pp. 399416.
    27. 27)
    28. 28)
      • 24. Guo, Y., Hu, S., Qi, X., Xiang, J., Sun, W.: ‘Voltammetric detection of adenosine-5′-diphosphate with a carbon paste electrode modified by a hydroxyl functionalized imidazolium-based ionic liquid’, Acta Chim. Slov., 2012, 59, (1), pp. 129135.
    29. 29)
    30. 30)
    31. 31)
http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2013.0050
Loading

Related content

content/journals/10.1049/iet-nbt.2013.0050
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading