Your browser does not support JavaScript!
http://iet.metastore.ingenta.com
1887

access icon free Protein characterisation of Brosimum gaudichaudii Trécul latex and study of nanostructured latex film formation

Brosimum gaudichaudii Tréc. (Moraceae) is a common Brazilian Cerrado plant known by its pharmaceutical industry relevance. The authors investigated the latex protein components and potential biotechnological applications. Some protein fragments had their sequences elucidated, presenting similarities to jacalin and Kunitz-type trypsin inhibitors. Amino acid residue modifications were found, such as glutamine N-terminal residue cyclisation into pyroglutamic acid residue, and mass differences corresponding to hexoses and N-acetylhexosamine presence. The latex was used to produce a nanoscale structured film, which presented an increased attraction and reduced adhesion behaviours. The film presented high homogeneity, as observed by low nanoroughness values, probably because of its intrinsic components, such as the jacalin-like protein that has known agglutination properties. The immobilised Kunitz-type trypsin inhibitor presence in the latex film allow us to point out to applications related to this inhibition, as in active food packaging, since these peptidase inhibitors are able to inhibit pests and microorganism proliferation.

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
    8. 8)
    9. 9)
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)
    18. 18)
    19. 19)
    20. 20)
    21. 21)
    22. 22)
    23. 23)
    24. 24)
    25. 25)
    26. 26)
    27. 27)
    28. 28)
    29. 29)
    30. 30)
    31. 31)
    32. 32)
    33. 33)
    34. 34)
    35. 35)
    36. 36)
    37. 37)
    38. 38)
    39. 39)
    40. 40)
    41. 41)
    42. 42)
      • 25. Licastro, F., Davis, L.J., Morini, M.C.: ‘Lectins and superantigens: membrane interactions of these with T lymphocytes affect immune responses’, Int. J. Biochem., 1993, 25, (6), pp. 845852 (doi: 10.1016/0020-711X(93)90239-B).
    43. 43)
      • 20. Caygill, J.C.: ‘Sulfhydryl plant proteases’, Enz. Microb. Technol., 1979, 1, (4), pp. 233242 (doi: 10.1016/0141-0229(79)90042-5).
    44. 44)
      • 31. Yu, L., Vizel, A., Huff, M.B., et al: ‘Investigation of N-terminal glutamate cyclization of recombinant monoclonal antibody in formulation development’, J. Pharmacol. Biomed. Anal., 2006, 42, (4), pp. 455463 (doi: 10.1016/j.jpba.2006.05.008).
    45. 45)
      • 22. Neidlema, S.L.: ‘Enzymes in the food industry a backward glance’, Food Technol., 1991, 45, (1), pp. 8891.
    46. 46)
      • 30. Busby, W.H.Jr., Quackenbush, G.E., Humm, J., et al: ‘An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides’, J. Biol. Chem., 1987, 262, (18), pp. 85328536.
    47. 47)
      • 52. Barbosa, E.F., Silva, L.P.: ‘Nanoscale characterization of synthetic polymeric porous membranes: scrutinizing their stiffness, roughness, and chemical composition’, J. Membr. Sci., 2012, 407–408, pp. 128135 (doi: 10.1016/j.memsci.2012.03.025).
    48. 48)
      • 43. Moussaoui, A.E., Nijs, M., Paul, C., et al: ‘Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defense mechanism’, Cell Mol. Life Sci., 2001, 58, (4), pp. 556570 (doi: 10.1007/PL00000881).
    49. 49)
      • 41. Konno, K., Hirayma, C., Nakamura, M., et al: ‘Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex’, Plant J., 2004, 37, (3), pp. 370378 (doi: 10.1046/j.1365-313X.2003.01968.x).
    50. 50)
      • 33. Joanitti, G.A., Freitas, S.M., Silva, L.P.: ‘Proteinaceous protease inhibitors: structural features and multiple functional faces’, Curr. Enzyme Inhib., 2006, 2, (3), pp. 199217 (doi: 10.2174/157340806777934801).
    51. 51)
      • 17. Lewinsohn, T.M.: ‘The geographical distribution of plant latex’, Chemoecology, 1991, 2, (1), pp. 6468 (doi: 10.1007/BF01240668).
    52. 52)
      • 12. Agrawal, A., Konno, K.: ‘Latex: a model for understanding mechanisms, ecology, and evolution of plant defense against herbivory’, Annu. Rev. Ecol. Evol. Syst., 2009, 40, pp. 311331 (doi: 10.1146/annurev.ecolsys.110308.120307).
    53. 53)
      • 3. Monteiro, V.F.F., Mathias, L., Vieira, I.J.C., et al: ‘Prenylated coumarins, chalcone and new cinnamic acid and dihydrocinnamic acid derivatives from Brosimum gaudichaudii’, J. Braz. Chem. Soc., 2002, 13, (2), pp. 281287 (doi: 10.1590/S0103-50532002000200023).
    54. 54)
      • 11. Hunter, J.: ‘Reconsidering the functions of latex’, Trees-Struct. Funct., 1994, 9, (1), pp. 15 (doi: 10.1007/BF00197862).
    55. 55)
      • 27. Pratap, J.V., Jeyaprakash, A.A., Rani, P.G., et al: ‘Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-α-D-mannose: implication to the generation of carbohydrate specificity’, J. Mol. Biol., 2002, 317, (2), pp. 237247 (doi: 10.1006/jmbi.2001.5432).
    56. 56)
      • 23. Engqvist, C., Forsberg, S., Norgren, M., et al: ‘Interactions between single latex particles and silica surfaces studied with AFM’, Coll. Surf. A, Physicochem. Eng. Aspects, 2007, 302, (1–3), pp. 197203 (doi: 10.1016/j.colsurfa.2007.02.032).
    57. 57)
      • 54. McNeil, L.E., Grimsditch, M.: ‘Elastic moduli of muscovite mica’, J. Phys. Condens. Mater., 1993, 5, pp. 16811692. (doi: 10.1088/0953-8984/5/11/008).
    58. 58)
      • 29. Gidrol, X., Chrestin, H., Tan, H.L., et al: ‘Hevein; a lectin-like protein from Hevea brasiliensis (rubber–tree) is involved in the coagulation of latex’, J. Biol. Chem., 1994, 269, (12), pp. 92789283.
    59. 59)
      • 7. Agra, M.F., Silva, K.N., Basílio, I.J.L.D., et al: ‘Survey of medicinal plants used in the region northeast of Brazil’, Rev. Bras. Farmacogn., 2008, 18, (3), pp. 472508 (doi: 10.1590/S0102-695X2008000300023).
    60. 60)
      • 2. Almeida, S.P., Proença, C.E.B., Sano, S.M., et al: ‘Cerrado: espécies vegetais úteis’ (Embrapa-CPAC, Brasília, Brazil, 1998).
    61. 61)
    62. 62)
    63. 63)
    64. 64)
      • 37. Moutim, V., Silva, L.G., Lopes, M.T.P., et al: ‘Spontaneous processing of peptides during coagulation of latex from Carica papaya, Plant Sci., 1999, 142, (2), pp. 115121 (doi: 10.1016/S0168-9452(98)00226-X).
    65. 65)
      • 15. Snook, M.E.: ‘Characterization and quantification of hexadecyl, octadecyl and eicosyl esters of p-coumaric acid in the vine and root latex of sweet potato [Ipomoea batatas (L.) Lam.]’, J. Agric. Food. Chem., 1994, 42, (11), pp. 25892595 (doi: 10.1021/jf00047a041).
    66. 66)
      • 21. Dupaigne, P.: ‘Some industrial applications of fruit components’, Fruits, 1973, 28, pp. 305318.
    67. 67)
      • 49. Vanderhoff1, J.W., Bradford, E.B., Carrington, W.K.: ‘The transport of water through latex films’, J. Polym. Sci., Polym. Symp., 1973, 41, (1), pp. 155174 (doi: 10.1002/polc.5070410116).
    68. 68)
      • 26. Peumans, W.J., Van Damme, E.J.M.: ‘Lectins as plant defense proteins’, Plant Physiol., 1995, 109, (2), pp. 347352 (doi: 10.1104/pp.109.2.347).
    69. 69)
      • 18. Palhares, D., De Paula, J.E., Silveira, C.: ‘Morphology of stem and subterranean system of Brosimum gaudichaudii (Moraceae)’, Acta Bot. Hung., 2006, 48, (1/2), pp. 8910 (doi: 10.1556/ABot.48.2006.1-2.12).
    70. 70)
      • 35. Christopher, M.E., Miranda, M., Major, I.T., et al: ‘Gene expression profiling of systemically wound-induced defenses in hybrid poplar’, Planta, 2004, 219, (6), pp. 936947 (doi: 10.1007/s00425-004-1297-3).
    71. 71)
      • 9. Anderson, T.F., Voorhees, T.F.: ‘Psoralen photochemotherapic of cutaneous disorders’, J. Ann. Rev. Pharmacol. Toxicol., 1980, 20, pp. 235257 (doi: 10.1146/annurev.pa.20.040180.001315).
    72. 72)
      • 42. Odani, S., Yokokawa, Y., Takeda, H., et al: ‘The primary structure and characterization of carbohydrate chains of the extracellular glycoprotein proteinase inhibitor from latex of Carica papaya, Eur. J. Biochem., 1996, 241, (1), pp. 7782 (doi: 10.1111/j.1432-1033.1996.0077t.x).
    73. 73)
      • 36. Philippe, R.N., Ralph, S.G., Kulheim, C., et al: ‘Poplar defense against insects: genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar kunitz-type protease inhibitor family’, New Phytol., 2009, 184, (4), pp. 865884 (doi: 10.1111/j.1469-8137.2009.03028.x).
    74. 74)
      • 55. Charmeau, J.Y.: ‘Effects of film structure on mechanical and adhesion properties of latex films’, Int. J. Adhes. Adhes., 1997, 17, (2), pp. 169176 (doi: 10.1016/S0143-7496(96)00034-6).
    75. 75)
      • 32. Seifert, F., Schulz, K., Koch, B., et al: ‘Glutaminyl cyclases display significant catalytic proficiency for glutamyl substrates’, Biochemistry, 2009, 48, (50), pp. 1183111833 (doi: 10.1021/bi9018835).
    76. 76)
      • 13. Gomes, M.T.R., Teixeira, R.D., Lopes, M.T.P., et al: ‘X-ray crystal structure of CMS1MS2: a high proteolytic activity cysteine proteinase from Carica candamarcensis, Amino Acids, 2012, 43, (6), pp. 23812391 (doi: 10.1007/s00726-012-1318-7).
    77. 77)
      • 4. Steiner, D., Bedin, V., Moraes, M.B., et al: ‘Vitiligo’, An. Bras. Dermatol., 2004, 79, (3), pp. 335351 (doi: 10.1590/S0365-05962004000300010).
    78. 78)
      • 50. Barbosa, E.F., Silva, L.P.: ‘Nanoscale analyses of modified polypropylene microtubes internal surface: an approach covering topographical and force spectroscopic parameters’, Surf. Inter. Anal., 2013, 45, pp. 17211726 (doi: 10.1002/sia.5313).
    79. 79)
      • 16. Romaniuc Neto, S., Wanderley, M.G.L.: ‘Flor fanerogâmica da reserva do Parque Estadual das Fonte do Ipiranga’, Hoehnea, 1992, 19, pp. 165169.
    80. 80)
      • 14. Dawson, C.R.: ‘The toxic principle of poison ivy and related plants’, J. Chem. Educ., 1943, 20, (9), pp. 448 (doi: 10.1021/ed020p448).
    81. 81)
      • 47. Lin, F., Meier, D.J.: ‘A study of latex film formation by atomic force microscopy. Film formation vs rheological properties: theory and experiment’, Langmuir, 1996, 12, (11), pp. 27742780 (doi: 10.1021/la951554w).
    82. 82)
      • 28. Young, N.M., Johnston, R.A., Szabo, A.G., et al: ‘Homology of the D-galactose-specific lectins from Artocarpus integrifolia and Maclura pomifera and the role of an unusual small polypeptide subunit’, Arch. Biochem. Biophys., 1989, 270, (2), pp. 596603 (doi: 10.1016/0003-9861(89)90542-0).
    83. 83)
      • 8. Pozetti, C.L.: ‘Contribuição ao estudo químico do Brosimum gaudichaudii Trécul’, Rev. Fac. Farm. Odont. de Araraquara, 1969, 3, (2), pp. 125223.
    84. 84)
      • 51. Barbosa, E.F., Silva, L.P.: ‘Nanoscale approaches over polypropylene plastic surfaces: an alternative tool for quality control checking’, in Barbosa, E.F., Silva, L.P. (Eds.): ‘Polypropylene: synthesis, applications and environmental concerns’ (NovaScience Publishers, 2012, 1st edn.), pp. 343357.
    85. 85)
      • 53. Vanlandingham, M.R., Villarrubia, J.S., Gutherie, W.F., et al: ‘Nanoindentation of polymers: An overview’. Macromol. Symp., 2001, vol. 167, pp. 1543.
    86. 86)
      • 44. Azarkan, M., Dibiani, R., Goormachtigh, E., et al: ‘The papaya kunitz-type trypsin inhibitor is a highly stable β-sheet glycoprotein’, Biochim. Biophys. Acta, 2006, 1764, (6), pp. 10631072 (doi: 10.1016/j.bbapap.2006.02.014).
    87. 87)
      • 34. Hollick, J.B., Gordon, M.P.: ‘Transgenic analysis of a hybrid poplar pound-inducible promoter reveals developmental patterns of expression similar to that of -storage protein cenes’, Plant Physiol., 1995, 109, (1), pp. 7385 (doi: 10.1104/pp.109.1.73).
    88. 88)
      • 1. Santos, K., Kinoshita, L.S.: ‘Flora arbustivo-arbórea do fragmento de floresta estacional semidecidual do Ribeirão Cachoeira, Município de Campinas, SP’, Acta Bot. Bras., 2003, 17, (3), pp. 325341 (doi: 10.1590/S0102-33062003000300001).
    89. 89)
      • 5. Macedo, M., Ferreira, A.R.: ‘Plantas medicinais usadas para tratamentos dermatológicos em comunidades da Bacia do Alto Paraguai, Mato Grosso’, Rev. Bras. Farmacogn., 2004, 14, pp. 4044 (doi: 10.1590/S0102-695X2004000300016).
    90. 90)
      • 48. Wang, Y., Juhu, D., Winnik, M.A., et al: ‘Atomic force microscopy study of latex film formation’, Langmuir, 1992, 8, (3), pp. 760762 (doi: 10.1021/la00039a004).
    91. 91)
      • 19. Silva, J.R.A., Amaral, A.C.F., Silveira, C.V., et al: ‘Quantitative determination by HPLC of iridoids in the bark and latex of Himatanthus sucuuba, Acta Amazônica, 2007, 37, (1), pp. 119122.
    92. 92)
      • 46. Goh, M.C., Juhu, D., Leung, O., et al: ‘Annealing effects on the surface structure of latex films studied by atomic force microscopy’, Langmuir, 1993, 9, (5), pp. 13191322 (doi: 10.1021/la00029a027).
    93. 93)
      • 38. Franco, O.L., Rigden, D.J., Melo, F.R., et al: ‘Plant α-amylase inhibitors and their interaction with insect α-amylases structure, function and potential for crop protection’, Eur. J. Biochem., 2002, 269, (2), pp. 397412 (doi: 10.1046/j.0014-2956.2001.02656.x).
    94. 94)
      • 10. Farrell, B.D., Dussourd, D.E., Mitter, C.: ‘Escalation of plant defense: do latex and resin canals spur plant diversification’, Am. Nat., 1991, 138, (4), pp. 881900 (doi: 10.1086/285258).
    95. 95)
      • 40. Kabir, S., Daar, A.S.: ‘The composition and properties of jacalin, a lectin of diverse applications obtained from jackfruit (Artocarpus heterophyllus) seeds’, Immunol. Invest., 1994, 23, (3), pp. 167188 (doi: 10.3109/08820139409087798).
    96. 96)
      • 39. Major, I.T., Constabel, C.P.: ‘Functional analysis of the kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores’, Plant Physiol., 2008, 146, (3), pp. 888903 (doi: 10.1104/pp.107.106229).
    97. 97)
      • 6. Agra, M.F., França, P.F., Barbosa-Filho, J.M.: ‘Synopsis of the plants known as medicinal and poisonous in northeast of Brazil’, Rev. Bras. Farmacogn., 2007, 17, (1), pp. 114140 (doi: 10.1590/S0102-695X2007000100021).
    98. 98)
      • 45. Christopher, A.J.: ‘Adsorption of charged latex particles on mica studied by atomic force microscopy’, J. Coll. Int. Sci., 1995, 179, (2), pp. 587599.
    99. 99)
      • 24. Sankaranarayanan, R., Sekar, K., Banerjee, R., et al: ‘A novel mode of carbohydrate recognition in jacalin, a Moraceae lectin with a B-prism fold’, Nature Struct. Biol., 1996, 3, (7), pp. 596603 (doi: 10.1038/nsb0796-596).
http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2013.0042
Loading

Related content

content/journals/10.1049/iet-nbt.2013.0042
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading
This is a required field
Please enter a valid email address