Incorporation of ligand–receptor binding-site models and transistor-based sensors for resolving dissociation constants and number of binding sites
- Author(s): Yu-Lin Wang 1 ; Chih-Cheng Huang 1 ; Yen-Wen Kang 1
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View affiliations
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Affiliations:
1:
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
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Affiliations:
1:
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
- Source:
Volume 8, Issue 1,
March 2014,
p.
10 – 17
DOI: 10.1049/iet-nbt.2013.0031 , Print ISSN 1751-8741, Online ISSN 1751-875X
Ligand–receptor binding site model is used to elucidate the binding affinity between ligands and receptors, with transistor-based sensors. AlGaN/GaN high electron mobility transistors (HEMTs) were immobilised with antibodies and human immunodeficiency virus type 1 reverse transcriptase enzymes to detect peptides and human immunodeficiency virus drugs, respectively. The signals generated by the sensors because of the binding of the ligands to the receptors were fitted into the binding-site models and analysed. The dissociation constants of the ligand–receptor pairs and the number of the binding sites on the receptors were revealed. The results are very consistent with the data reported by the other methods from the literatures. The incorporation of the HEMTs and the binding-site models is demonstrated to be useful for studying the mechanism of the biomolecular interaction and the application for quick and cost-effective drug developments.
Inspec keywords: III-V semiconductors; enzymes; wide band gap semiconductors; dissociation; high electron mobility transistors; aluminium compounds; biosensors; molecular biophysics; drugs; gallium compounds; biochemistry
Other keywords: dissociation constants; binding affinity; antibodies; peptides; AlGaN-GaN; ligand-receptor binding site models; high electron mobility transistors; human immunodeficiency virus type 1 reverse transcriptase enzymes; biomolecular interaction; transistor-based sensors; HEMT; cost-effective drug developments; human immunodeficiency virus drugs
Subjects: Biomolecular interactions, charge transfer complexes; Physical chemistry of biomolecular solutions and condensed states; Biosensors; Decomposition reactions (pyrolysis, dissociation, and group ejection); Other field effect devices; Biosensors; Biological engineering and techniques
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