access icon free Spatially selective release of aptamer-captured cells by temperature mediation

Isolation of cells from heterogeneous biological samples is critical in both basic biological research and clinical diagnostics. Affinity-based methods, such as those that recognise cells by binding antibodies to cell membrane biomarkers, can be used to achieve specific cell isolation. Microfluidic techniques have been employed to achieve more efficient and effective cell isolation. By employing aptamers as surface-immobilised ligands, cells can be easily released and collected after specific capture. However, these methods still have limitations in cell release efficiency and spatial selectivity. This study presents an aptamer-based microfluidic device that not only achieves specific affinity cell capture, but also enables spatially selective temperature-mediated release and retrieval of cells without detectable damage. The specific cell capture is realised by using surface-patterned aptamers in a microchamber on a temperature-control chip. Spatially selective cell release is achieved by utilising a group of microheater and temperature sensor that restricts temperature changes, and therefore the disruption of cell–aptamer interactions, to a design-specified region. Experimental results with CCRF-CEM cells and sgc8c aptamers have demonstrated the specific cell capture and temperature-mediated release of selected groups of cells with negligible disruption to their viability.

Inspec keywords: microfluidics; biothermics; biomembranes; cellular biophysics; temperature sensors; organic compounds; bioMEMS

Other keywords: surface-immobilised ligand; microchamber; cell membrane biomarker; specific cell capture; efficient cell isolation; aptamer-based microfluidic device; microfluidic technique; sgc8c aptamer; cell–aptamer interaction disruption; effective cell isolation; temperature sensor; spatially selective temperature-mediated retrieval; cell spatial selectivity; aptamer-captured cell spatially selective release; microheater sensor; specific affinity cell capture; cell release efficiency; binding antibody; heterogeneous biological sample; experimental result; spatially selective temperature-mediated release; CCRF-CEM cell; temperature mediation; cell recognition; basic biological research; cell collection; surface-patterned aptamer; clinical diagnostics; temperature change restriction; detectable cell damage; spatially selective cell release; affinity-based method; design-specified region; temperature-control chip

Subjects: Biological engineering and techniques; Cellular biophysics; Biomedical engineering; Natural and artificial biomembranes; Biophysical instrumentation and techniques; MEMS and NEMS device technology; Micromechanical and nanomechanical devices and systems; Thermal variables measurement; Biomedical measurement and imaging; Biothermics

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