access icon free Theoretical and experimental studies on ionic currents in nanopore-based biosensors

Novel generation of analytical technology based on nanopores has provided possibilities to fabricate nanofluidic devices for low-cost DNA sequencing or rapid biosensing. In this paper, a simplified model was suggested to describe DNA molecule's translocation through a nanopore, and the internal potential, ion concentration, ionic flowing speed and ionic current in nanopores with different sizes were theoretically calculated and discussed on the basis of Poisson–Boltzmann equation, Navier–Stokes equation and Nernst–Planck equation by considering several important parameters, such as the applied voltage, the thickness and the electric potential distributions in nanopores. In this way, the basic ionic currents, the modulated ionic currents and the current drops induced by translocation were obtained, and the size effects of the nanopores were carefully compared and discussed based on the calculated results and experimental data, which indicated that nanopores with a size of 10 nm or so are more advantageous to achieve high quality ionic current signals in DNA sensing.

Inspec keywords: Boltzmann equation; biosensors; biological fluid dynamics; molecular biophysics; Poisson equation; nanoporous materials; nanofluidics; nanobiotechnology; DNA; Navier-Stokes equations; ionic conductivity

Other keywords: ionic current; nanopore-based biosensors; translocation; ionic flowing speed; Nernst-Planck equation; ion concentration; Navier-Stokes equation; Poisson-Boltzmann equation; nanofluidic devices; low-cost DNA sequencing

Subjects: MEMS and NEMS device technology; Biosensors; Biosensors; Biological engineering and techniques; Micromechanical and nanomechanical devices and systems

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
    8. 8)
    9. 9)
      • 20. International Standard, ISO 13319:2007(E), ISO, Geneva, Switzerland, 2007.
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)
    18. 18)
    19. 19)
    20. 20)
    21. 21)
    22. 22)
    23. 23)
    24. 24)
      • 21. Gu, L.Q., Cheley, S., Bayley, H.: ‘Electroosmotic enhancement of the binding of a neutral molecule to a transmembrane pore’. Proc. of the National Academy of Sciences, USA, 2003, vol. 100, pp. 1549815503.
    25. 25)
    26. 26)
    27. 27)
      • 14. Zhao, Q., Wang, Y., Dong, J.J.: ‘Nanopore-based DNA analysis via graphene electrodes’, J. Nanomater., 2012, 39, pp. 318950.
    28. 28)
    29. 29)
    30. 30)
    31. 31)
    32. 32)
    33. 33)
    34. 34)
http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2013.0017
Loading

Related content

content/journals/10.1049/iet-nbt.2013.0017
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading