Theoretical and experimental studies on ionic currents in nanopore-based biosensors
- Author(s): Lei Liu 1, 2 ; Chu Li 1 ; Jian Ma 1 ; Yingdong Wu 1 ; Zhonghua Ni 1 ; Yunfei Chen 1
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View affiliations
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Affiliations:
1:
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China;
2: Suzhou Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Suzhou Research Institute of Southeast University, Suzhou 215123, People's Republic of China
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Affiliations:
1:
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China;
- Source:
Volume 8, Issue 4,
December 2014,
p.
247 – 256
DOI: 10.1049/iet-nbt.2013.0017 , Print ISSN 1751-8741, Online ISSN 1751-875X
Novel generation of analytical technology based on nanopores has provided possibilities to fabricate nanofluidic devices for low-cost DNA sequencing or rapid biosensing. In this paper, a simplified model was suggested to describe DNA molecule's translocation through a nanopore, and the internal potential, ion concentration, ionic flowing speed and ionic current in nanopores with different sizes were theoretically calculated and discussed on the basis of Poisson–Boltzmann equation, Navier–Stokes equation and Nernst–Planck equation by considering several important parameters, such as the applied voltage, the thickness and the electric potential distributions in nanopores. In this way, the basic ionic currents, the modulated ionic currents and the current drops induced by translocation were obtained, and the size effects of the nanopores were carefully compared and discussed based on the calculated results and experimental data, which indicated that nanopores with a size of 10 nm or so are more advantageous to achieve high quality ionic current signals in DNA sensing.
Inspec keywords: Boltzmann equation; biosensors; biological fluid dynamics; molecular biophysics; Poisson equation; nanoporous materials; nanofluidics; nanobiotechnology; DNA; Navier-Stokes equations; ionic conductivity
Other keywords: ionic current; nanopore-based biosensors; translocation; ionic flowing speed; Nernst-Planck equation; ion concentration; Navier-Stokes equation; Poisson-Boltzmann equation; nanofluidic devices; low-cost DNA sequencing
Subjects: MEMS and NEMS device technology; Biosensors; Biosensors; Biological engineering and techniques; Micromechanical and nanomechanical devices and systems
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