%0 Electronic Article %A Arpita Gupta %+ Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India %A Shital R. Bonde %+ Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India %A Swapnil Gaikwad %+ Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India %A Avinash Ingle %+ Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India %A Aniket K. Gade %+ Department of Biology, Utah State University, Logan, UT 84322-5305, USA %A Mahendra Rai %+ Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India %+ Laboratório de Química Biológica, Instituto de Química – Unicamp Cidade Universitária ‘Zefferino Vaz’, Barão Geraldo-CEP 13083-970, Caixa Postal 6150 Campinas, SP, Brazil %K Microsporum canis %K L. inermis %K zeta potential %K antibiotics %K Lawsonia inermis mediated synthesis %K Candida albicans %K M. canis %K microbial resistance %K capped nanoparticle formation %K antimicrobial nanogel formulation %K intermediate complex formation %K P. acne %K C. albicans %K Trichophyton mentagrophytes %K antimicrobial nanoparticle gel %K UV-vis spectrophotometer %K bioreduction %K Ag %K antimicrobial activity %K silver nanoparticles %K T. mentagrophytes %K nanoparticle tracking %K bactericidal agents %K disc diffusion method %K Fourier transform infrared spectroscopy %K human pathogenic fungi %K biosynthesis route %K leaf extract %K UV-visible spectrophotometer %K Propioniabacterium acne %K transmission electron microscopy %K fungicidal agents %K drug-resistant microorganisms %K chemical agents %X Lawsonia inermis mediated synthesis of silver nanoparticles (Ag-NPs) and its efficacy against Candida albicans, Microsporum canis, Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag-NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO3. The Ag-NPs were characterized by Ultraviolet-Visible (UV–Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag-NPs. The antimicrobial activity of Ag-NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag-NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag-NPs may be an answer to the drug-resistant microorganisms. %@ 1751-8741 %T Lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel %B IET Nanobiotechnology %D September 2014 %V 8 %N 3 %P 172-178 %I Institution of Engineering and Technology %U https://digital-library.theiet.org/;jsessionid=2g20n29dfnq3i.x-iet-live-01content/journals/10.1049/iet-nbt.2013.0015 %G EN