Your browser does not support JavaScript!
http://iet.metastore.ingenta.com
1887

access icon free Ionic selectivity of nystatin A1 confined in nanoporous track-etched polymer membrane

© The Institution of Engineering and Technology
Received 01/03/2013, Accepted 17/06/2013, Revised 10/06/2013, Published 15/08/2013

The hybrid biological/polymeric solid-state nanopore membrane offers several opportunities to combine the advantage of biological channel (selectivity) and material (robustness). Based on this technology, the challenge is to obtain selective ionic exchange membranes, with no energy intake. The direct insertion of an ionic channel inside a nanopore should be a promise solution. Here, the authors report a hybrid nanopore based on nystatin A1 confinement in commercial nanopore membrane. Ionic transport and selectivity studies show that the hybrid nanopores exhibit mainly an anionic behaviour, on the contrary to biological conditions. However, the order of magnitude between the different ratios of permeation of several cationic species is retained even if the blocking of divalent cation is not totally proved.

Inspec keywords: etching; permeability; biomembrane transport; nanomedicine; polymers; drugs; bioelectric phenomena; biomedical materials; nanoporous materials

Other keywords: divalent cation; ionic selectivity; ionic exchange membrane; nanoporous track-etched polymer membrane; ionic transport; ionic channel; commercial nanopore membrane; direct insertion; cationic species; nystatin A1 confinement; permeation ratios; biological channel; hybrid biological-polymeric solid-state nanopore membrane; anionic behaviour; selectivity study

Subjects: Nanotechnology applications in biomedicine; Natural and artificial biomembranes; Biomedical materials; Biological transport; cellular and subcellular transmembrane physics; Bioelectricity

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
    8. 8)
    9. 9)
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)
    18. 18)
    19. 19)
    20. 20)
    21. 21)
      • 19. Kourennyi, D.E., Barnes, S.: ‘Depolarization-induced calcium channel facilitation in rod photoreceptors is independent of G proteins and phosphorylation’, J. Neurophysiol., 2000, 84, (1), pp. 133–138.
    22. 22)
      • 18. Stefanovic, J., Jakovljevic, D., Gojgic-Cvijovic, G., et al: ‘Synthesis, characterization, and antifungal activity of nystatingum arabic conjugates’, J. Appl. Polymer Sci., 2013, 127, (6), pp. 4736–4743 (doi: 10.1002/app.38084).
    23. 23)
      • 5. Kocer, A., Tauk, L., Dejardin, P.: ‘Nanopore sensors: from hybrid to abiotic systems’, Biosens. Bioelectron., 2012, 38, (1), pp. 1–10 (doi: 10.1016/j.bios.2012.05.013).
    24. 24)
      • 2. Lockless, S.W., Zhou, M., MacKinnon, R.: ‘Structural and thermodynamic properties of selective ion binding in a K+ channel’, Plos Biology, 2007, 5, (5), pp. 1079–1088 (doi: 10.1371/journal.pbio.0050121).
    25. 25)
      • 22. Balme, S., Picaud, F., Kraszewski, S., et al: ‘Controlling potassium selectivity and proton blocking in a hybrid biological/solid-state polymer nanoporous membrane’, Nanoscale, 2013, 5, pp. 3961–3968 (doi: 10.1039/c3nr00564j).
    26. 26)
      • 15. Janot, J.M., Boissiere, M., Thami, T., et al: ‘Adsorption of Alexa-Labeled Bt toxin on mica, glass, and hydrophobized glass: study by normal scanning confocal fluorescence’, Biomacromolecules, 2010, 11, (6), pp. 1661–1666 (doi: 10.1021/bm100313n).
    27. 27)
      • 7. Fologea, D., Gershow, M., Ledden, B., et al: ‘Detecting single stranded DNA with a solid state nanopore’, Nano Lett., 2005, 5, (10), pp. 1905–1909 (doi: 10.1021/nl051199m).
    28. 28)
      • 9. Balme, S., Janot, J.-M., Berardo, L., et al: ‘New bioinspired membrane made of a biological ion channel confined into the cylindrical nanopore of a solid-state polymer’, Nano Lett., 2011, 11, pp. 712–716 (doi: 10.1021/nl103841m).
    29. 29)
      • 3. Gouaux, E., MacKinnon, R.: ‘Principles of selective ion transport in channels and pumps’, Science, 2005, 310, (5753), pp. 1461–1465 (doi: 10.1126/science.1113666).
    30. 30)
      • 16. Balme, S., Janot, J.M., Dejardin, P., et al: ‘Potentialities of confocal fluorescence for investigating protein adsorption on mica and in ultrafiltration membranes’, J. Membr. Sci., 2006, 284, (1–2), pp. 198–204 (doi: 10.1016/j.memsci.2006.07.034).
    31. 31)
      • 1. MacKinnon, R.: ‘Potassium channels and the atomic basis of selective ion conduction (Nobel lecture)’, Angew. Chem., Int. Ed., 2004, 43, (33), pp. 4265–4277 (doi: 10.1002/anie.200400662).
    32. 32)
      • 11. Akaike, N., Harata, N.: ‘Nystatin perforated-patch recording and its applications to analyses of intracellular mechanisms’, Jpn. J. Physiol., 1994, 44, (5), pp. 433–473 (doi: 10.2170/jjphysiol.44.433).
    33. 33)
      • 10. Pradeep, H., Rajanikant, G.K.: ‘Nanochannels: biological channel analogues’, IET Nanobiotechnol., 2012, 6, (2), pp. 63–70 (doi: 10.1049/iet-nbt.2011.0033).
    34. 34)
      • 12. Thiele, D., Kraszewski, S., Balme, S., et al: ‘Structure and ionic selectivity of a hybrid polyene/artificial polymer solid state membrane’, Soft Matter, 2013, 9, pp. 684–691 (doi: 10.1039/c2sm26703a).
    35. 35)
      • 8. Apel, P.Y.: ‘Track etching technique in membrane technology’, Radiat. Meas., 2001, 34, pp. 559–566 (doi: 10.1016/S1350-4487(01)00228-1).
    36. 36)
      • 17. Pickard, W.: ‘Generalizations of th Goldman-Hodgkin-Katz equation’, Math. Biosci., 1976, 30, pp. 99–111 (doi: 10.1016/0025-5564(76)90018-3).
    37. 37)
      • 4. Kowalczyk, S.W., Blosser, T.R., Dekker, C.: ‘Biomimetic nanopores: learning from and about nature’, Trends Biotechnol., 2011, 29, (12), pp. 607–614 (doi: 10.1016/j.tibtech.2011.07.006).
    38. 38)
      • 20. Russell, J.M., Eaton, D.C., Brodwick, M.S.: ‘Effects of nystatin on membrane conductance and internal ion activities in aplysia neurons’, J. Membr. Biol., 1977, 37, (2), pp. 137–156.
    39. 39)
      • 21. Marty, A., Finkelstein, A.: ‘Pores formed in lipid bilayer membranes by nystatin – differences in its one-sided and 2-sided action’, J. Gen. Physiol., 1975, 65, (4), pp. 515–526 (doi: 10.1085/jgp.65.4.515).
    40. 40)
      • 13. Cass, A., Finkelstein, A., Krespi, V.: ‘The ion permeability induced in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B’, J. Gen. Physiol., 1970, 56, pp. 100 (doi: 10.1085/jgp.56.1.100).
    41. 41)
      • 6. Hall, A.R., Scott, A., Rotem, D., et al: ‘Hybrid pore formation by directed insertion of alpha-haemolysin into solid-state nanopores’, Nat. Nanotechnol., 2010, 5, (12), pp. 874–7 (doi: 10.1038/nnano.2010.237).
    42. 42)
      • 14. Borisova, M.P., Brutyan, R.A., Ermishkin, L.N.: ‘Mechanism of anion-cation selectivity of amphotericin-B channels’, J. Membr. Biol., 1986, 90, (1), pp. 13–20 (doi: 10.1007/BF01869681).
http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2013.0014
Loading

Related content

content/journals/10.1049/iet-nbt.2013.0014
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading
Print this page
This is a required field
Please enter a valid email address