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Probing the interaction of bovine haemoglobin with gold nanoparticles

Probing the interaction of bovine haemoglobin with gold nanoparticles

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The interaction between gold nanoparticles (GNPs) and bovine haemoglobin (BHb) was studied by ultraviolet–visible (UV–Vis) absorption, circular dichroism (CD) and fluorescence spectroscopic techniques. The UV–Vis absorption spectrum demonstrated that there was interaction between GNPs and BHb, but no direct interaction between GNPs and haem groups of BHb. The fluorescence data revealed that GNPs effectively quenched the intrinsic fluorescence of BHb via static quenching. The binding of GNPs to BHb occurred at a single site. The binding process was a spontaneous molecular interaction procedure, in which hydrophobic force and hydrogen bonds played a major role. The alternations of protein secondary structure in the presence of GNPs were also determined by CD spectroscopy. This work is helpful to understand the interaction mechanism of GNPs with haemoglobin, which can guide the applications of GNPs in biomedicine.

Inspec keywords: circular dichroism; nanomedicine; hydrogen bonds; visible spectra; hydrophobicity; ultraviolet spectra; molecular biophysics; molecular configurations; gold; fluorescence; radiation quenching; nanoparticles; proteins

Other keywords: haem groups; hydrophobic force; hydrogen bonds; fluorescence spectroscopy; circular dichroism; ultraviolet-visible absorption spectra; gold nanoparticles; static quenching; binding process; bovine haemoglobin; biomedicine; spontaneous molecular interaction; Au; protein secondary structure

Subjects: Ultraviolet molecular spectra; Nanotechnology applications in biomedicine; Biomolecular structure, configuration, conformation, and active sites; Visible molecular spectra; Biomolecular interactions, charge transfer complexes; Biomolecular dynamics, molecular probes, molecular pattern recognition; Molecular fluorescence and phosphorescence spectra; Interactions with radiations at the biomolecular level; Optical activity, optical rotation, circular dichroism in molecules; Molecular bond strengths, dissociation energies, hydrogen bonding

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http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2011.0029
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