RT Journal Article
A1 Y. Ruan
A1 W. Yu
A1 F. Cheng
A1 X. Zhang
A1 T. Rao
A1 Y. Xia
A1 S. Larré

PB iet
T1 Comparison of quantum-dots- and fluorescein-isothiocyanate-based technology for detecting prostate-specific antigen expression in human prostate cancer
JN IET Nanobiotechnology
VO 5
IS 2
SP 47
OP 51
AB Quantum dots (QDs) are a new class of fluorescent labelling for biological and biomedical applications. In this study, the authors evaluated the sensitivity and stability of quantum-dots-based immunolabelling, in comparison with the conventional fluorescein-isothiocyanate-based immunolabelling (FITC), for detecting prostate-specific antigen (PSA) expression in human prostate cancer. The authors' data revealed that the two methods had similar sensitivity in differential display of the PSA expression correlated with tumour stage and grade (κ=0.88, p<0.001). Moreover, the intensity of QDs fluorescence remain stable for 10 days after conjugation to the PSA protein in 97% of the cases and more than 1 month in 92% of the cases, although the FITC fluorescence became undetectable after 6 min for all cases.
K1 biomedical application
K1 PSA protein
K1 quantum-dots-based immunolabelling
K1 fluorescent labelling
K1 biological application
K1 tumour stage
K1 fluorescein-isothiocyanate-based immunolabelling
K1 time 6 min
K1 human prostate cancer
K1 prostate-specific antigen expression
K1 FITC fluorescence
K1 time 10 day
DO https://doi.org/10.1049/iet-nbt.2010.0016
UL https://digital-library.theiet.org/;jsessionid=dac32l3uk75n.x-iet-live-01content/journals/10.1049/iet-nbt.2010.0016
LA English
SN 1751-8741
YR 2011
OL EN