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Comparison of quantum-dots- and fluorescein-isothiocyanate-based technology for detecting prostate-specific antigen expression in human prostate cancer

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Abstract

Quantum dots (QDs) are a new class of fluorescent labelling for biological and biomedical applications. In this study, the authors evaluated the sensitivity and stability of quantum-dots-based immunolabelling, in comparison with the conventional fluorescein-isothiocyanate-based immunolabelling (FITC), for detecting prostate-specific antigen (PSA) expression in human prostate cancer. The authors' data revealed that the two methods had similar sensitivity in differential display of the PSA expression correlated with tumour stage and grade (κ=0.88, p<0.001). Moreover, the intensity of QDs fluorescence remain stable for 10 days after conjugation to the PSA protein in 97% of the cases and more than 1 month in 92% of the cases, although the FITC fluorescence became undetectable after 6 min for all cases.

References

    1. 1)
      • 1 onward link is available for this reference.
      • CrossRef
    2. 2)
      • 1 onward link is available for this reference.
      • CrossRef
    3. 3)
      • 1 onward link is available for this reference.
      • CrossRef
    4. 4)
      • 1 onward link is available for this reference.
      • CrossRef
    5. 5)
      • 1 onward link is available for this reference.
      • CrossRef
    6. 6)
      • 1 onward link is available for this reference.
      • CrossRef
    7. 7)
      • 1 onward link is available for this reference.
      • CrossRef
    8. 8)
      • D.F. Gleason , M. Tannebaum . (1977) Histologic grading and clinical staging of prostatic carcinoma’, Urologic pathology: the prostate.
    9. 9)
      • O.H. Beahrs , D.E. Henson , R.V.P. Hutter , B.J. Kennedy . (1992) AJCC manual for staging of cancer.
    10. 10)
      • 1 onward link is available for this reference.
      • CrossRef
    11. 11)
      • 1 onward link is available for this reference.
      • CrossRef
    12. 12)
      • 1 onward link is available for this reference.
      • CrossRef
    13. 13)
      • R. Stege , M. Grande , K. Carlström , B. Tribukait , Å. Pousette . Prognostic significance of tissue prostate-specific antigen in endocrine-treated prostate carcinomas. Clin. Cancer Res. , 160 - 165
    14. 14)
      • 1 onward link is available for this reference.
      • CrossRef
    15. 15)
      • 1 onward link is available for this reference.
      • CrossRef
    16. 16)
      • F. Cheng , W. Yu , X. Zhang , Y. Ruan . Quantum-dot-based technology for sensitive and stable detection of prostate stem cell antigen expression in human transitional cell carcinoma. Int. J. Biol. Markers , 271 - 276
    17. 17)
      • 1 onward link is available for this reference.
      • CrossRef

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content/journals/10.1049/iet-nbt.2010.0016
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