Adhesion, proliferation and differentiation of pluripotent stem cells on multi-walled carbon nanotubes
Adhesion, proliferation and differentiation of pluripotent stem cells on multi-walled carbon nanotubes
- Author(s): J. Holy ; E. Perkins ; X. Yu
- DOI: 10.1049/iet-nbt.2010.0014
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- Author(s): J. Holy 1 ; E. Perkins 2 ; X. Yu 3
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View affiliations
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Affiliations:
1: Department of Anatomy, Microbiology and Pathology, University of Minnesota, School of Medicine-Duluth, Duluth, USA
2: Department of Biomedical Sciences, Mercer University School of Medicine, Savannah Campus, Savannah, USA
3: Department of Mechanical and Industrial Engineering, University of Minnesota, Duluth, USA
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Affiliations:
1: Department of Anatomy, Microbiology and Pathology, University of Minnesota, School of Medicine-Duluth, Duluth, USA
- Source:
Volume 5, Issue 2,
June 2011,
p.
41 – 46
DOI: 10.1049/iet-nbt.2010.0014 , Print ISSN 1751-8741, Online ISSN 1751-875X
© The Institution of Engineering and Technology
Published
This article studies the adhesion, growth and differentiation of stem cells on carbon nanotube matrices. Glass coverslips were coated with multi-walled carbon nanotube (MWNT) thin films using layer-by-layer self-assembling techniques. Pluripotent P19 mouse embryonal carcinoma stem cells were seeded onto uncoated or MWNT-coated glass coverslips and either maintained in an undifferentiated state or induced to differentiate by the addition of retinoic acid. The authors found that cell adhesion was increased on the MWNT-coated glass surfaces, and that the expression patterns of some differentiation markers were altered in cells grown on MWNTs. The results suggest that MWNTs will be useful in directing pluripotent stem cell differentiation for tissue engineering purposes.
Inspec keywords: cellular biophysics; adhesion; nanobiotechnology; tissue engineering; self-assembly; carbon nanotubes; cancer
Other keywords:
Subjects: Nanotechnology applications in biomedicine; Self-assembly in nanofabrication; Cellular biophysics; Biomedical engineering
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