Lateral field excited film bulk acoustic resonator for detection of protein–ligand interactions
Presented is a lateral field excited film bulk acoustic resonator for real-time sensing of protein–ligand interaction using the biotin–streptavidin system as a model. The film bulk acoustic resonator works at 2 GHz in shear mode. For biosensing, the biotin was immobilised on one face of the resonator as the probe. The resonant frequency of the biotin-immobilised device drops rapidly at first and gradually reaches equilibrium when exposed to the streptavidin solutin due to the biotin–streptavidin interaction. Consequently, the lateral field excited film bulk acoustic resonator can be used to study the kinetics of protein–ligand interaction without the use of labelling or molecular tags. The proposed device shows promising applications for disease diagnostics, prognosis, and drug discovery.
