SERS combines high sensitivity and selectivity with practicality through the use of microfluidics. SERS based techniques are cost effective and rapid which makes them an ideal biosensor for the detection of pathogens.

Chapter Contents:

• 10.1 Introduction
• 10.2 Current technologies for pathogen detection
• 10.3 Enzyme-linked immunosorbent assay (ELISA)
• 10.4 Polymerase chain reaction (PCR)
• 10.5 Biosensors
• 10.5.1 Overview
• 10.5.2 Biorecognition techniques
• 10.5.3 Antibodies
• 10.5.4 Enzymes
• 10.5.5 Nucleic acids
• 10.6 Transduction techniques
• 10.6.1 Electrochemical
• 10.6.2 Piezoelectric
• 10.7 Sample preparation
• 10.8 Optical biosensors
• 10.8.1 Surface plasmon resonance (SPR)
• 10.9 Fluorescence
• 10.9.1 Auto-fluorescent protein (AFP)
• 10.9.2 Bioluminescence resonance energy transfer
• 10.9.3 Quantum dots
• 10.10 Surface enhanced Raman spectroscopy (SERS)
• 10.11 Other methods
• 10.11.1 Interferometry
• 10.11.2 Optical fibre
• 10.11.3 Carbon nanotubes
• 10.12 Conclusions and future prospects
• Acknowledgements
• References

Inspec keywords: microfluidics; biosensors; bioMEMS; surface enhanced Raman scattering; optical sensors

Other keywords: surface enhanced Raman scattering; microfluidics; food borne pathogen detection; SERS; optical biosensor

Subjects: Biomedical engineering; Biosensors; MEMS and NEMS device technology; Biomedical measurement and imaging; Sensing and detecting devices; Biosensors; Sensing devices and transducers; Applied fluid mechanics; Microfluidics and nanofluidics; Micromechanical and nanomechanical devices and systems